The detection and significance of Foxp3+ regulatory T cells, Th17 in peripheral blood of non-small cell lung cancer patients
10.3760/cma.j.issn.0254-5101.2012.07.017
- VernacularTitle:非小细胞肺癌患者外周血Foxp3+调节性T细胞和Th17的检测与临床意义
- Author:
Sha LI
;
Yan LI
;
Jing LIANG
;
Xiaolin LIU
- Publication Type:Journal Article
- Keywords:
Non-small cell lung cancer;
Foxp3+;
Regulatory T cells(Tr);
Th17
- From:
Chinese Journal of Microbiology and Immunology
2012;32(7):642-646
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the Foxp3+ regulatory T cells (TrFoxp3+),Th17,TrFoxp3+/Th17 and related cell factors level in peripheral blood of non-small cell lung cancer(NSCLC) patients,and to explore the relationships about TrFoxp3+,Th17 with occurrence and development of lung cancer and whether there are imbalance of TrFoxp3+/Th17.Methods The proportions of TrFoxp3+,Th17 cells and the level of related cell factors such as TGF-β,IL-17,IL-23 were determined respectively by flow cytometry and ELISA in peripheral blood of 18 healthy people and 26 patients with NSCLC.Results The proportions of TrFoxp3+,Th17 and TrFoxp3+/Th17 in peripheral blood with NSCLC were higher than healthy controls(P ＜0.05).The proportion of Th17 cells from NSCLC patients was positively correlated with that of TrFoxp3+(r =0.81,P＜0.05).Comparing TrFoxp3+/Th17 among different TNM stages in NSCLC patients:TrFoxp3+/Th17 of in Ⅳ stage was obvious higher than that of in Ⅰ-Ⅱ stage,Ⅲ stage(P＜0.05).The level of TGF-β,IL-17,IL-23 was higher in NSCLC patients than that in healthy controls,the ratio of TGF-β in the advanced stage group/healthy controls was higher in the early and middle group/healthy controls (P＜0.05).Conclusion TrFoxp3+,Th17 and TrFoxp3+/Th17 in NSCLC patients were higher than healthy people,moreover TrFoxp3+/Th17 in advanced stage increased significantly.The results may imply that there are certain relationships between the imbalance of TrFoxp3+/Th17 and immunosuppression and occurrence and development of NSCLC as well as the regulation of their cytokines.
