Depressive effect of multiple-siRNA-APRIL in SW480 cell
10.3760/cma.j.issn.0254-5101.2012.07.013
- VernacularTitle:串联siRNA干扰沉默结肠癌SW480细胞APRIL基因表达
- Author:
Meihong LU
;
Haiquan LI
;
Guihua WANG
;
Jingchun WANG
;
Weifeng DING
;
Huimin WANG
- Publication Type:Journal Article
- Keywords:
RNA interference (RNAi);
Multiple siRNA;
A proliferation-inducing ligand;
Colon carcinoma
- From:
Chinese Journal of Microbiology and Immunology
2012;32(7):629-633
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prove the remarkable inhibitive effect of multiple siRNAs targeting a proliferation-inducing ligand (APRIL) on the human colorectal cancer cell.Methods We constructed a multiple short hairpin RNA(shRNA) expression vector containing four shRNAs (pG4) as well as four single one (pGsh644,pGsh1451,pGsh1938,pGsh2231) against APRIL gene in SW480 cell,and then transfected them into the human colorectal cancer cell line by cationic liposome.Ultimately,SW480 were screened by EGFP to obtain expression cell lines.APRIL expression levels including mRNA and APRIL protein were detected after transfected with all different kinds of vectors.Results A multiple shRNA expression vector containing four shRNAs (pG4) and four single ones were successfully constructed.Four single vectors (pGsh644,pGsh1451,pGsh1938,pGsh2231) and the multiple siRNAs expression vector (pG4) all decreased the APRIL mRNA by 56.2％,49.5％ ;50.9％,49.2％ and 79.3％.And APRIL protein expression was also remarkably reduced,especially by multiple siRNAs expression vector(87.5％).Conclusion Multiple siRNAs expression vector produced a more significant knockdown effect of APRIL than the vectors containing only one APRIL shRNA.What we found suggested us using the vector containing multiple shRNA to silence the expression of APRIL might be exploited as a novel therapeutic strategy for tumors.
