The effect of hyperbaric oxygen combined with drug treatment on the cell cycle of colon carcinoma Lovo cells
10.3760/cma.j.issn.0254-1424.2011.011.005
- VernacularTitle:高压氧联合细胞周期特异性药物对结肠腺癌Lovo细胞的杀伤作用
- Author:
Lida ZHANG
;
Xiaoqiang TIAN
;
Wei ZHANG
;
Lin YANG
;
Aiguo WANG
;
Ping HUANG
;
Xia QIAN
- Publication Type:Journal Article
- Keywords:
Hyperbaric oxygenation;
Fluorouracil;
Cell proliferation;
Colon carcinoma cells
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2011;33(11):815-818
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of hyperbaric oxygen(HBO)combined with 5-fluorouracil (5-FU)chemotherapy on colon carcinoma Lovo cells.Methods Lovo ceils were exposed to 0.20 MPa HBO combined with 5-FU at different concentrations.The cell cycle was monitored with flow cytometry,and cell proliferation was detected using a methylthiazol tetrazolium test(MTT).Results ①After exposure to HBO,phase accumulation of Lovo cells was significantly higher than in the control group,and the accumulation of Lovo cells exposed to HBO after 24 h was significantly higher than at 12 and 48 hours.②There was difference between HBO(0.20 MPa,post 24 h)+ 5-FU(≤8 pM)group and 5-FU(≤8 μM,12 h)group on the inhibition of cells proliferation(P ＜0.05),and significan difference could be seen between the two groups after treatment for 48 h(P ＜ 0.01); ③There was no significant difference between HBO + 5-FU group and 5-FU group treatment with the concentration(16,32,64 μM)of 5-FU(P ＞ 0.05)for 12 h ;however,the difference could be seen after treatment for 48 h between the two groups(P ＜ 0.05).Conclusion The lethal effect on Lovo cells of 5-FU can be enhanced by exposure to 0.20 MPa HBO,especially with low concentrations of 5-FU.
