The effect of 50 Hz sinusoidal electromagnetic fields on the proliferation of the osteosarcoma cell line MG-63
10.3760/ema.j.issn.0254-1424.2011.011.004
- VernacularTitle:50Hz正弦电磁场对骨肉瘤MG-63细胞增殖的影响
- Author:
Lin SHANG
;
Yang LIU
;
Mingyu SONG
;
Jizhe YU
;
Hua WU
- Publication Type:Journal Article
- Keywords:
Sinusoidal electromagnetic fields;
Osteosarcoma cells;
Proliferation;
Cell cycle
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2011;33(11):812-814
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the biological effect of 50 Hz sinusoidal electromagnetic fields at 1 mT on the proliferation of the human osteosarcoma cell line MG-63.Methods Osteosarcrma MG-63 cells were divided into control and experimental groups.The control group was incubated without an electromagnetic field; the experimental group was incubated in a 50 Hz,1.0 mT sinusoidal electromagnetic field.On the 2nd,4th and 6th day,their proliferation was determined using a cell counting kit-8(CCK-8)assay.Variations in the cell cycle were detected with flow cytometry(FCM).Semi-quantitative reverse transcription-polymerase chain reaction(RT-PCR)was used to measure cyclin B1 and cyclin D1 mRNA.Results Compared with the control group,proliferation of the experimental group cells was reduced significantly.The percentage of cells at G0-G1 phase increased,and the mRNA expression of cyclin B1 and cyclin D1 was significantly reduced.Conclusions A 50 Hz sinusoidal electromagnetic field at 1.0 mT can inhibit the proliferation of osteosarcoma cell line MG-63 significantly.
