Protective effect of aliskiren on type 2 diabetic nephropathy in db/db mice
10.3760/cma.j.issn.1001-7097.2011.11.012
- VernacularTitle:阿利吉仑对2型糖尿病db/db小鼠肾脏损伤的保护作用
- Author:
Guangyu ZHOU
;
Xuesong SU
;
Feng DU
;
Detian LI
- Publication Type:Journal Article
- Keywords:
Renin-angiotensin system;
Diabetic nephropathy;
Aliskiren;
Renin inhibitor
- From:
Chinese Journal of Nephrology
2011;27(11):844-849
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of aliskiren on type 2 diabetic nephropathy in db/db mice.Methods Eight-week old db/db and db/m mice were subjected to right nephrectomy to hasten the development of diabetic nephropathy.At age of 16 weeks,they were divided into four groups:db/m group (normal control),db/db group ( diabetic control),db/db+ A3 group (db/db mice treated with aliskiren 3 mg·kg-1d-1) and db/db+A25 group (db/db mice treated with aliskiren 25 mg kg-1 d-1).Body weight,blood glucose,glycosylated hemoglobin,proteinuria and systolic blood pressure were measured before and after treatment.After treatment,renal histological examination by PAS staining,TGF-β1 and PAI-1 protein by ELISA,expression of ColⅣ and FN protein by immunofluorescence,mRNA expression of TGF-β1,PAI-1,ColⅣ,FN and renin by real time PCR,renin activity and angiotensin Ⅱ level by radioimmunoassay were performed.Results Treatment for 4 weeks of aliskiren at a dose of 25 mg kg-1 d-1 markedly decreased urinary albumin excretion,glomerulosclerosis and suppressed synthesis of TGF-β1,PAI-1,Col Ⅳ,FN without inducing significantl change in systolic blood pressure,compared to those of db/db group (all P＜0.05).Aliskiren also suppressed renin activity and angiotensin Ⅱ level in renal cortex (all P＜0.05).Conclusion Aliskiren protects against type 2 diabetic nephropathy.
