Effects of tacrolimus on the secretion of interleukin-6 (IL-6), soluble interleukin-2 receptor (sIL-2) and expression of IL-6 and sIL-2R mRNA by a human lymphoma cell line Jurkat

VernacularTitle:他克莫司对人Jurkat淋巴瘤细胞分泌IL-6和sIL-2R及表达IL-6和sIL-2R mRNA的影响
Author: Junling ZHANG ; Cuiyan YAN ; Danfeng SUO ; Xiuliang MA
Publication Type:Journal Article
From: Chinese Journal of Dermatology 2012;45(10):745-746
CountryChina
Language:Chinese
Abstract: Objective To evaluate the effects of tacrolimus on the secretion of IL-6 and sIL-2R as well as the expression of IL-6 and sIL-2R mRNA by lymphocytes.Methods Jurkat human lymphoma cells were cultured and treated with tacrolimus of different concentrations.Enzyme linked immunosorbent assay was performed to determine the levels of IL-6 and sIL-2R in the supernatant of Jurkat cells at 48 hours after treatment with tacrolimus of 0,10,102,103 and 104 nmol/L,and real time reverse transcription PCR to measure the expression of IL-6 mRNA and sIL-2R mRNA of Jurkat cells at 48 hours after treatment with tacrolimus of 102 nmol/L.Results Tacrolimus of 102 - 104 nmol/L could suppress the secretion of IL-6 and sIL-2R from Jurkat cells (all P＜ 0.05),with a more marked suppressing effect achieved by the use of tacrolimus at 103 - 104 nmol/L.The expressions of IL-6 and sIL-2R mRNA from Jurkat cells were downregulated by tacrolimus of 102 nmol/L (both P ＜ 0.05).Conclusion Tacrolimus at certain concentrations could downregulate the secretion of IL-6 and sIL-2R as well as the expression of IL-6 and sIL-2R mRNA by lymphocytes.