Single nucleotide polymorphisms in the open reading frame 26 (ORF26) gene of human herpesvirus 8 (HHV-8) in Kaposi's sarcoma
10.3760/cma.j.issn.0412-4030.2011.11.017
- VernacularTitle:人疱疹病毒8型ORF26单核苷酸多态性研究
- Author:
Xiujuan WU
;
Yan SHI
;
Ying JIN
;
Dezhi ZHANG
;
Xiongming PU
- Publication Type:Journal Article
- From:
Chinese Journal of Dermatology
2011;44(11):805-807
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo study the single nucleotide polymorphisms(SNPs) in the ORF26 gene of HHV-8 in Kaposi's sarcoma(KS),and to assess their correlations with the clinical phenotype and mucosal invasion of KS.MethodsHHV-8 DNA was extracted with phenol-chloroform-isoamyl alcohol from paraffin-embedded tissue specimens obtained from 32 cases of KS(including 26 classic and 6 AIDS-related KS).The ORF26 gene of HHV-8 was amplified by nested-PCR followed by bidirectional sequencing.The software DNAStar and program Clustal W were used to assess the SNPs in the ORF26 gene.Statistical analysis was carried out by using the Fisher's exact probability test.ResultsHHV-8 DNA was detected in 30 of the 32 tissue specimens,and in all of the 6 AIDS-related specimens.The predominant SNPs were 981 T/C(n =12),1086 C/T(n =12) and 1139 A/C(n =12) in the ORF26 gene of the 30 strains of HHV-8.No significant difference was observed in the distribution of SNPs in ORF26 between different phenotypes of KS or between KS with and without mucosal invasion.ConclusionThe ORF26 SNPs of HHV-8 seem unrelated to the clinical phenotypes or mucosal invasion of KS.