Detection and analysis of protein and mRNA expressions of pituitary tumor transforming gene (PTTG) and basic fibroblast growth factor (bFGF) in cutaneous squamous cell carcinoma tissue

VernacularTitle:皮肤鳞状细胞癌组织中垂体瘤转化基因和碱性成纤维细胞生长因子蛋白及mRNA的检测与分析
Author: Yonghua XIA ; Caifeng ZHANG ; Sujuan LI ; Dong LIU ; Jinmei GUO ; Min LI ; Zhanguo LI ; Dandan FU ; Zhongwei TIAN
Publication Type:Journal Article
Keywords: Carcinoma; squamous cell; Oncogenes; Fibroblast growth factor 2
From: Chinese Journal of Dermatology 2011;44(11):789-792
CountryChina
Language:Chinese
Abstract: ObjectiveTo investigate the role and clinical pathological significance of PTTG and bFGF in cutaneous squamous cell carcinoma(CSCC).MethodsTissue specimens were collected from the lesions of 42 patients with CSCC and normal skin of 42 normal human controls.The protein and mRNA expressions of PTTG and bFGF were detected by immunohistochemistry and in situ hybridization in these specimens respectively.ResultsA significant increase was observed in the positive expression rates of PTTG and bFGF proteins[64.3％(27/42) vs.11.9％(5/42),73.8％(31/42) vs.21.4％(9/42),both P＜ 0.05] and mRNA [59.5％(25/42) vs.7.1％(3/42),75.0％(29/42) vs.16.7％(7/42),both P＜ 0.05] in the CSCC tissue specimens than in the control specimens.The protein and mRNA expressions of PTTG were positively correlated with those of bFGF(both P ＜ 0.05),and closely correlated with histological grade of CSCC (both P ＜0.05).ConclusionThe high expression of PTTG and bFGF may be associated with the initiation of CSCC.