Effects of hydrogen-rich medium on lipopolysaccharide-induced release of inflammatory cytokines from RAW264.7 macrophages
10.3760/cma.j.issn.0254-1416.2012.08.025
- VernacularTitle:富氢液对内毒素诱导RAW264.7细胞炎性因子释放的影响
- Author:
Hongguang CHEN
;
Keliang XIE
;
Huanzhi HAN
;
Weina WANG
;
Guolin WANG
;
Yonghao YU
- Publication Type:Journal Article
- Keywords:
Hydrogen;
Endotoxemia;
Macrophages;
Inflammation
- From:
Chinese Journal of Anesthesiology
2012;32(8):988-990
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of hydrogen-rich medium on lipopolysaccharide (LPS)-induced release of inflammatory cytokines from murine macrophage RAW264.7 cells.Methods The RAW264.7cells were cultured in DMEM culture medium containing 10％ fetal bovine serum.The cells were seeded in 6-well plates (3 ml/hole) with a density of 2 × 106/ml and randomly divided into 4 groups (n =24 each):control group (group A),hydrogen-rich medium group (group B),LPS group (group C) and LPS + hydrogen-rich medium group (group D).Group A received no treatment.LPS 1 μg/ml was added to the medium in groups C and D,while the cells were incubated with 0.6 mmol/L hydrogen-rich medium simultaneously in groups B and D.Six wells in each group were chosen at 6,12.24 and 48 h of incubation,and the supernatant was collected to detect the concentrations of TNF-α,IL-1β and IL-10.Six wells in each group were chosen at 6 and 12 h of incubation for determination of heme oxygenase-1 (HO-1) expression in the cells by Western blot.Results Compared with group A,the concentrations of TNF-α,IL-1β and IL-10 in the supernatant were significantly increased,and the expression of HO-1 was up-regulated in group C (P ＜ 0.05).Compared with group C,the concentrations of TNF-α and IL-1β in the supernatant were significantly decreased,the concentration of IL-10 in the supernatant was significantly increased,and the expression of HO-1 was up-regulated in group D (P ＜ 0.05).Conclusion Hydrogen-rich medium can inhibit the release of pro-inflammatory cytokines and promote the release of anti-inflammatory cytokines from RAW264.7 cells via up-regulating HO-1 expression.
