Effect of artificial circadian rhythm of melatonin on postoperative cellular immune function in patients undergoing gynecological operation
10.3760/cma.j.issn.0254-1416.2012.08.008
- VernacularTitle:人工建立褪黑素节律对妇科手术病人术后细胞免疫功能的影响
- Author:
Yongwei CHENG
;
Tiehu YE
;
Xiangyang GUO
- Publication Type:Journal Article
- Keywords:
Melatonin;
Immunity,cellular;
Gynecologic surgical procedures
- From:
Chinese Journal of Anesthesiology
2012;32(8):933-935
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of artifictal circadian rhythm of melatonin on the postoperative cellular immune function in patients undergoing gynecological operation Methods Eighteen ASA Ⅰ or Ⅱ patients,aged 25-50 yr,weighing 45-80 kg,scheduled for elective gynecological operation,were randomly divided into 3 groups (n =6 each):control group (group C),placebo control group (group P) and melatonin group (group M).In group M.melatonin 6 mg was given orally at 10 min before lights-out (21:00) on 1 day before operation,on the day of operation and on 1 day after operation,while placebo was given orally instead of melatonin in group P.The operation was performed under epidural anesthesia.Patient-controlled epidural analgesia with ropivacaine was used for postoperative analgesia.VAS score was maintained ＜ 5.Blood samples were collected from the peripheral vein at 1 day before operation (baseline),the end of operation and 1 day after operation to measure CD4+,CD8+ and CD3+ cell count by flow cytometry.The ratio of the number of CD4+ cells to the number of CD8+ cells was calculated.Results There were no significant differences in the number of CD4+,CD8+ and CD3 + cells and ratio of the number of CD4 + cells to the number of CD8 + cells between groups C,P and M (P ＞0.05).Conclusion Artificial circadian rhythm of melatonin exerts no influence on the postoperative cellular immune function in patients undergoing gynecological operation.
