Effect of midazolam on human sperm motility in vitro
10.3760/cma.j.issn.0254-1416.2012.08.001
- VernacularTitle:咪达唑仑对人离体精子能动性的影响
- Author:
Fang CAI
;
Jintao WANG
;
Ailin LUO
;
Liu YANG
;
Shiyong LI
;
Zheng LIU
;
Yong ZHANG
- Publication Type:Journal Article
- Keywords:
Midazolam;
Sperm motility;
In vitro
- From:
Chinese Journal of Anesthesiology
2012;32(8):909-911
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of midazolam on human sperm motility in vitro.Methods Sperm samples were obtained from normal adults and prepared with discontinuous percoll gradient centrifugation technique.The samples were randomly divided into 3 groups (n =10 each):control group and 2 midazolam groups.The samples were incubated with normal saline in control group and with midazolam with the final concentrations of 5 or 1 μg/ml in 2 midazolam groups.The samples were incubated for 60 min in an airtight container at 37 ℃.Then human sperm motility was examined in vitro at 37 ℃ and analyzed by the computer-assisted sperm analysis at 10,30 and 60 min exposure to midazolam,including sperm motility (a + b)％,curvilinear velocity,straight line velocity,average path velocity,amplitude of lateral head displacement,beat-cross frequency,linearity,wobble,straightness,and mean angular displacement.Results There was no significant difference in the parameters of human sperm motility within each group and between groups (P ＞ 0.05).Conclusion Midazolam has no significant effect on human sperm motility in vitro.
