Cloning and characterization of 5'-upstream region of human phospholipase C-beta2 gene.
- Author:
Eun Sook YUN
1
;
Seung Jae LEE
;
Myung Jong KIM
;
Sung Ho RYU
;
Pann Ghill SUH
Author Information
1. Dept. Life Science, Pohang University of Science and Technology, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
phospholipase C-b2;
promoter;
cloning;
transcription
- MeSH:
Aspergillus Nuclease S1/metabolism;
Base Sequence;
Cells, Cultured;
Chloramphenicol O-Acetyltransferase/metabolism;
Cloning, Molecular;
Conserved Sequence;
Gene Deletion;
Isoenzymes/*chemistry/*genetics;
Molecular Sequence Data;
Mutagenesis, Site-Directed;
Phospholipase C/*chemistry/*genetics;
Promoter Regions (Genetics);
Protein Binding;
Support, Non-U.S. Gov't;
Transcription, Genetic;
Transfection
- From:Experimental & Molecular Medicine
2001;33(2):76-82
- CountryRepublic of Korea
- Language:English
-
Abstract:
5'-upstream region of the phospholipase C-beta2 gene, 810 bp, was cloned and characterized. S1 nuclease mapping and primer extension analyses revealed that a single transcriptional start site locates at 284 nucleotides upstream from the beginning of translation. The 5-upstream region lacks both TATA motif and typical initiator sequence, but retains GC-rich segment. Two putative regulatory regions, a negative region (-636/-588) and a positive region (-98/ -13) were identified in the upstream region of PLC-beta2 gene. We suggest that the transcription of PLC-beta2 may be regulated by binding of regulatory proteins to the negative and/or positive regulatory regions located in the upstream of the gene.
