Change and significance of osteoprotegerin/receptor activator of NF-κB ligand during differentiation of rat arterial smooth muscle cells into the osteoblast-like cells
10.3760/cma.j.issn.0254-9026.2012.10.022
- VernacularTitle:大鼠主动脉中膜平滑肌细胞向成骨样细胞转化中骨保护素和核转录因子κB受体配体的变化及意义
- Author:
Bin NIE
;
Siming GUAN
;
Xin FANG
;
Shaoqiong ZHOU
- Publication Type:Journal Article
- Keywords:
Osteoprotegerin;
NF-kappa B;
Monocytes,Smooth muscle;
Tunica media;
Osteoblasts
- From:
Chinese Journal of Geriatrics
2012;31(10):899-902
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the change and significance of osteoprotegerin(OPG)/receptor activator of NF κB ligand(RANKL)during the process of rat arterial smooth muscle cells (SMC) to differentiate subgroups:7 d (early) subgroup and 14 d (late) subgroup.into osteoblast-like cells.Methods The rat arterial SMCs were divided randomly into the SMC group,atorvastatin group,and osteoblast-like group.Each group was also divided into 2.The osteoblast-like group was given β-glycerophosphate and vitamin C in culture medium,the atorvastatin group was given both β-glycerophosphate,vitamin C and atorvastatin.Von Kossa staining,Ca2+ content assay,ALP activity assay and osteocalcin assayed with Western blot were used to check the level of calcification.Real-time PCR was used to check the mRNA expressions of OPG and RANKL.Results There was high expression of OPG (early 2.71 ±0.08,late 2.69 ±0.02) but no RANKL in the SMC group all the time.The expressions of OPG were increased in the early subgroup and decreased in late subgroup in the atorvastatin group (early 3.52±0.05,late 2.50±0.03) and osteoblast-like group (early 4.18±0.10,late 2.30 ± 0.11).And the expressions of RANKL were both increased in the atorvastatin group and osteoblast-like group dnring the calcification process (from 1.01 ± 0.19 to 2.40 ± 0.10,and from 1.70±0.07 to 3.22±0.11,respectively).Among the three groups,the ratio of OPG/RANKL was decreased with the increasing calcification level (F =52.93,2.33,both P＜0.05).And compared with the early subgroups,the ratio of OPG/RANKL in the late subgroups was also decreased along with the increase of calcification level in each group(F=38.71,1.74,both P＜0.05).Conclusions The ratio of OPG/RANKL has a negative correlation with the level of the osteoblast-like cells differentiation,and atorvastatin could inhibit the calcification.
