An enzymatic method for the detection of human serum albumin.
- Author:
Masood Ul Hassan JAVED
1
;
Saima N WAQAR
Author Information
1. Department of Biochemistry, The Aga Khan University Medical College, Karachi, Pakistan. masoodjaved@hotmail.com
- Publication Type:Original Article
- Keywords:
albumin;
human;
LDH-5;
serum
- MeSH:
Chemistry, Clinical/*methods;
Chromatography, Gel;
Human;
Isoenzymes/metabolism;
Lactate Dehydrogenase/metabolism;
Protein Binding;
Sepharose/chemistry;
Serum Albumin/*analysis
- From:Experimental & Molecular Medicine
2001;33(2):103-105
- CountryRepublic of Korea
- Language:English
-
Abstract:
Albumin is the most abundant protein in human serum. A dye-binding method is commonly used in clinical laboratories for its estimation using different types of dyes. However, all these dye methods were interfered by a variety of compounds. Here we present a method for the detection of albumin in human serum and other biological fluids. The principle is based on the fact that lactate dehydrogenase isoenzyme-5 (LDH-5) binds specifically to Dextran-Blue (DB). Albumin inhibits the binding of LDH-5 with DB. Absence of LDH activity in DB fraction after gel filtration indicates the presence of albumin in sample and vice versa.
