Peripheral blood Th17 and CD4 + CD25+ regulatory T cell levels and their correlations in patients with primary hepatocellular carcinoma
10.3760/cma.j.issn.1674-2397.2012.05.001
- VernacularTitle:原发性肝癌患者外周血Th17与CD4+CD25+调节性T细胞表达的相关性研究
- Author:
Jie LI
;
Wanhua REN
;
Jun SHI
;
Wei WU
;
Zhi CHEN
- Publication Type:Journal Article
- Keywords:
Liver neoplasms;
Th17 cells;
Regulatory T cell;
Immunomodulation
- From:
Chinese Journal of Clinical Infectious Diseases
2012;5(5):257-260
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the peripheral blood Th17 and CD4 + CD25 + regulatory T cell levels and their correlations in patients with primary hepatocellular carcinoma (PHC).Methods Peripheral blood samples were collected from 30 PHC patients and 25 healthy controls in the First Affiliated Hospital of Zhejiang University from June 2008 to May 2009.Mononuclear cells were isolated and the Th17 and CD4 + CD25 + regulatory T cells were detected by flow cytometry and compared between patients and controls by t test.Spearman test was performed to analyze the correlation of Th17 with CD4 + CD25 +regulatory T cell concentrations.Results The levels of Th17 and CD4 + CD25 + regulatory T cells in peripheral blood in healthy controls were (2.10 ± 0.87) ％ and (7.10 ± 2.32) ％ ; while those in PHC patients were (3.38±1.68)％ and (11.78±5.62)％ (t=3.640 and 4.162,P＜0.01).The level of Th17 cells was positively associated with that of CD4 + CD25 + regulatory T cells in PHC patients (r =0.821,P ＜0.01).Conclusion The levels of Th17 and CD4 + CD25 + regulatory T cells in peripheral blood are high in PHC patients and positively correlated with each other,which indicates that CD4 + CD25 + regulatory T cells may contribute to the disease progression and pathogenesis of carcinoma through inducing Th17 cells differentiation.
