Variation of RANKL/OPG in serum and T lymphocytes of patients with rheumatoid arthritis and their clinical significance
10.3760/cma.j.issn.1007-7480.2012.11.009
- VernacularTitle:RANKL/骨保护素在类风湿关节炎患者血清及T细胞上的表达及临床意义
- Author:
Yu WANG
;
Shengqian XU
- Publication Type:Journal Article
- Keywords:
Arthritis,rheumatoid;
NF-kappa B;
Flow cytometry;
Osteoprotegerin
- From:
Chinese Journal of Rheumatology
2012;16(11):754-758
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the positive expression level of CD3+ mRANK+ cells in the peripheral blood and the level of sRANKL and OPG in serum and culture supernatant of patients with rheumatoid arthritis (RA) and investigate the association between RANKL/RANK/OPG system and the destruction of RA.Methods Twenty-four RA patients and 20 healthy controls were collected in the Rheumatology and Immunology Department of the First Affiliated Hospital of Anhui Medical University.The peripheral blood mononuclear cell (PBMC) were extracted,subsequently stimulated in vitro with PHA for 72 hours.Flow cytometry (FCM) was used to detect the positive expression level of CD3+ mRANK+ cells in the peripheral blood,and ELISA was used to detect the level of sRANKL and OPG in serum and culture supernatant.Clinical and laboratory parameters were recorded.Multivariate linear regression and t-test were used to analyze the data from different groups.Results There was no statistical significant difference in the expression level of CD3+ mRANK+ cells [(14±4)％ vs (15±7)％,P＞0.05] in RA patients compared with that in healthy controls.Compared with healthy controls,the level of sRANKL [(133±49) vs (41 ±8) pmol/L,P＜0.05],OPG [(390±154) vs (222±38) pg/ml,P＜0.05] and sRANKL/OPG [(0.40 ±0.23) vs (0.19±0.05),P＜0.05] increased in the serum of RA patients.The level of sRANKL [(34±19) vs (17±5) pmol/l,P＜0.05] and sRANKL/OPG [(0.39±0.23) vs (0.19 ±0.05),P＜0.05] increased in culture supernatant of RA patients.Multivariate linear regression showed that there was a negative correlation between DAS28 and the level of OPG in serum in RA ((β)=-0.681,t=3.315,P=0.005).There was a positive correlation between anti-CCP and sRANKL/OPG ratio inserum in RA((β)=0.497,t=2.326,P=0.040).There was a negative correlation between mRANKL and the Sharp method ((β)=0,522,t=2.370,P=0.032).There was a negative correlation between HAQ and the Sharp method ((β)=0.559,t=2.536,P=0.023).Conclusion Abnormality of RANKL shedding of T lymphocytes may be another inmmune machanism for the bone destruction of RA,The level of mRANKL expressed by activated T cell is a risk factor for the severity of bone damage of RA.In the bone destruction of RA,Anti-CCP antibody and RANKL/RANK/OPG system may be closely related.
