The expression of TLR2 and high mobility group protein 1 in systemic lupus erythematosus
10.3760/cma.j.issn.1007-7480.2012.10.010
- VernacularTitle:Toll样受体2与高迁移率族蛋白1在系统性红斑狼疮患者中的表达
- Author:
Liping SUN
;
Yongming WU
;
Lili YANG
;
Liping MENG
- Publication Type:Journal Article
- Keywords:
Lupus erythematosus,systemic;
High mobility group protein 1;
TLRs;
Flow cytometry
- From:
Chinese Journal of Rheumatology
2012;16(10):688-691
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the pathogenesis and disease progression by detecting the expression of Serum High mobility group protein 1 (HMGB1) and TLR2 in monocytes of patients with systemic lupus erythematosus (SLE).Methods Forty patients with SLE were selected randomly,20 patients were in active disease group and others were in stable disease group.The expression of HMGB1 in the serum of these cases were detected by enzyme linked immunosorbent assay (ELISA) and TLR2 on CD14+ monocytes in the peripheral blood were detected by FCM.The correlation between these indexes and clinical,laboratory indexes about SLE were analyzed using one-way ANOVA,and Kruskal-Wallis test.Results The expression levels of HMGB-1 in serum was [(48.9±11.3) μg/L] in the active group,while that was [(14.8±1.9) μg/L] in the stable group was,and [(13.5±3.6) μg/L] in the control group.HMGB1 in the active SLE group was significantly higher (P＜0.05) when compared with that of the stable and control group.The expression of TLR2 in the peripheral blood mononuclear cells was [(96.7±1.3)％] in the active group,[(83.5±9.1)％] in the stable group,and [(83.3±9.9)％] in the control group TLR2 in the active SLE group was up-regulated when compared with the stable and control groups (P＞0.05).There were positive correlation between the serum levels of HMGB1and TLR2 in the peripheral blood mononuclear cells (r=0.551,P＜0.05).Conclusion The expression levels of HMGB-1 in serum and the expression of TLR2 in peripheral blood mononuclear cells may participate in the pathological processes of SLE.
