Immunologic properties of osteogenic differentiated bone mesenchymal stem cells

VernacularTitle:骨向分化骨髓间充质干细胞的免疫学特性研究
Author: Jun FANG ; Huazhuang LI ; Gang DING ; Guangzong ZHAO ; Kehai GAO ; Jingchun CHEN
Publication Type:Journal Article
Keywords: Bone marrow; Mesenchymal stem cells; Cell differentiation; Immunosuppression
From: Chinese Journal of Trauma 2012;28(9):838-841
CountryChina
Language:Chinese
Abstract: Objective To investigate the immunologic properties of osteogenic differentiated bone mesenchymal stem cells (BMSCs). Methods BMSCs were isolated from normal volunteers and induced in osteogenic medium for two weeks. Then,non-differentiated/osteogenic differentiated BMSCs were co-cultured with allogenic T cells and phytohemagglutinin (PHA).The proliferation of T cells was examined by MTT method.The concentrations of TGF-β1 in osteogenic differentiated BMSCs supernatants at week 2 and mixed lymphocytes reaction (MLR) supernatants at day 5 were determined by ELISA.Also,anti-TGF-β antibody was added into the MLR to detect the response of the mixed T cells. Results Non-differentiated and osteogenic differentiated BMSCs did not induce proliferation of the allogeneic T cells but both suppressed the proliferation of the T cells mediated by PHA.The TGF-β1 concentrations had significant elevation in the MLR.Anti-TGF-β antibody could counteract the immunosuppressive function of the osteogenic differentiated BMSCs. Conclusion Osteogenic differentiated BMSCs possess low immunogenicity and immunosuppressive property.