In vitro studies of the effects of high-fat dieton the expression of pancreatic acinar cells' IP3 and amylase release in rats
10.3760/cma.j.issn.1008-6315.2012.12.013
- VernacularTitle:高脂饮食对大鼠胰腺腺泡细胞三磷酸肌醇表达及淀粉酶释放影响的体外研究
- Author:
Mingxian YAN
;
Huaqing ZHAO
;
Yaru WANG
;
Xiaolong LI
;
Jing YANG
;
Wenqi WANG
;
Yiguo WANG
- Publication Type:Journal Article
- Keywords:
High-fat diet;
Pancreatic acinar cell;
1,4,5-trisphosphate;
Excrine;
Cholecystokinin
- From:
Clinical Medicine of China
2012;28(12):1276-1279
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of high-fat diet on pancreatic acinar cells' IP3 expression and CCK-induced amylase release in rats.Methods Male Wistar rats were divided into high-fat diet group and normal diet group,they were fed for 4 weeks.Blood triglycerides,cholesterol,amylase and glucose levels were determined by automatic biochemical analyzer.Pancreatic tissues were taken for histopathological observations.Pancreatic acinar cells were isolated and cultured,and intracellular inositol 1,4,5-trisphosphate (IP3) was detected using a commercial kit.Amylase release rates were measured after CCK-8 stimulation.Results The rats in high-fat diet group appeared hyperlipidemia,vacuolization of acinar cells and the lymphocytes appeared around the acinar cells can be seen on the pancreatic tissue pathology staining.The levels of IP3 in acinar cells of rats fed a high-fat diet were higher than that of normal rats [(31.807 ± 3.448) pmol/106 cells vs (24.632 ± 3.649) pmol/106 cells,t=7.479,P＜0.001];and amylase release rate in these rats'acinar cells were also higher than those of normal rats [when CCK-8=0.01 nmol/L:( 11.056 ±3.369)％ vs (7.354 ± 2.181) ％,t=3.912,P＜0.001;when CCK-8=1 nmol/L:( 13.854 ± 4.087 ) ％ vs (9.432 ±2.477) ％,t=3.939,P＜0.001 ) after CCK-8 stimulation in different concentrations.Additionally,there was a positive co-relationship between acinar cell's IP3 level and amylase release (r=0.896,P＜0.001 ).Conclusion Chronic high-fat diet induces hypersensitivity for pancreatic acinar cells' exocrine function,and IP3 as a signal molecule may play an important role in this process.
