Impact of ischemic stroke on the intestinal barrier function in dogs
10.3760/cma.j.issn.1674-635X.2012.04.008
- VernacularTitle:缺血性脑卒中对犬肠屏障功能的影响
- Author:
Yecheng LIU
;
Guizhen HE
;
Zhiwei QI
;
Jun XU
;
Huadong ZHU
;
Zhong WANG
;
Xuezhong YU
;
Sui MA
- Publication Type:Journal Article
- Keywords:
Ischemic stroke;
Intestinal barrier function;
Apoptosis;
Tight junction;
Occludin;
Zonula occludens-1
- From:
Chinese Journal of Clinical Nutrition
2012;20(4):234-237
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the impact of ischemic stroke on intestinal barrier changes in dogs.Methods Totally 20 mongrel dogs were divided into 2 groups by random number table with 10 in each.Double silicone cylinders measuring 1.1 mm in diameter and 8 mm in length were placed into their internal carotid arteries in all dogs of group A.Group B served as a control group and received sham operation.Light microscopy was performed for morphological measurement of intestinal epithelial cell.Immunohistochemistry was used to analysis the changes of protein zonula occludens-1(ZO-1)localizing at tight junction of intestinal epithelial cells.Results Ischemic stroke was confirmed by cranial CT scanning in all dogs of group A.Compared with the test results in group B,the occludin and Zo-1 protein levels in group A were significantly lower than those in group B(occludin:0.20 ±0.01 vs 0.22 ±0.01,P =0.007; ZO-1:0.20 ±0.01 vs 0.22 ±0.02,P =0.008).The apoptotic index in group A was significantly higher than in group B(29.04 ± 3.79 vs 6.44 ± 1.24,P =0.002).There was a positive correlation between occludin and ZO-1(R =0.71,P =0.02),and the apoptotic index was negatively correlated with levels of occludin,ZO-1(R =-0.91,P =0.00; R =-0.77,P =0.01).Light microscopy showed that the dogs in group A had intestinal mucousal injuries while no obvious change was detected in group B.Conclusions Dogs with ischemic stroke tend to develop intestinal barrier dysfunction,during which the destruction of tight junction plays a key role.The up-regulated apoptosis of intestinal epithelial cell constitutes one of the cellular bases of intestine injury.
