Effects of ulinastatin on toll-like receptor 4 signaling pathway in lung tissue of rats after lipopolysaccharide insult

VernacularTitle:乌司他丁对脂多糖诱导的大鼠肺组织Toll样受体4信号通路表达的影响
Author: Rihong HUANG ; Xianyao WAN
Publication Type:Journal Article
Keywords: Lipopolysaccharide; Ulinastatin; TLR4; NF-κB; Lung injury
From: Chinese Journal of Emergency Medicine 2012;21(11):1226-1229
CountryChina
Language:Chinese
Abstract: Objective To investigate the protective mechanisms of ulinastatin (UTI) in lung injury in septic rats.Methods Thirty healthy SD rats of clean grade were randomly (random number) divided into saline control group,lipopolysaccharide (LPS) group and lipopolysaccharide + ulinastatin (LPS + UTI) group (n =10,in each).The lung tissues were obtained for morphological observation at the 24th-hour after LPS injection.RT-PCR or Western blot,ELISA methods were applied to measure the expressions of Toll-like receptor 4 (TLR4),nuclear factor (NF)-κB,tumor necrosis factor (TNF)-α mRNA and their protein levels.Results LPS injection could result in lung injury.UTI could down-regulate the expressions of TLR4 mRNA (t =3.563,P =0.032),TNF-α mRNA (t =5.147,P =0.028),TLR4 protein (t =2.692,P =0.041),NF-κB protein (t =2.459,P =0.024) and TNF-α protein (t =3.336,P =0.037) in lung,and could in turn alleviate lung injury.Conclusions UTI showed protective effects on LPS induced lung injury,and one of the mechanisms was perhaps associated with the inhibition of TLR4-NF-κB signal transduction pathway.