Changes of myocardial ATPase activity and plasma TNF-α concentrations in rats with acute craniocerebral injury
10.3760/cma.j.issn.1671-0282.2011.12.009
- VernacularTitle:急性颅脑损伤大鼠心肌ATP酶活性和血浆TNF-α含量的变化
- Author:
Xiaomeng ZHU
;
Shide CHEN
- Publication Type:Journal Article
- Keywords:
Acute craniocerebral injury;
Myocardial injury;
Tumor necrosis factor-alpha;
ATPase;
Na + -K + -ATPase;
Ca2 + -ATPase;
Cardiac troponin;
Rat
- From:Chinese Journal of Emergency Medicine
2011;20(12):1259-1263
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes of myocardial ATPase activity and plasma tumor necrosis factor- alpha (TNF- a) levels in rats with acute craniocerebral injury (ACI).Methods Sixtyfour male SD rats were randomly (random number) divided into sham group and model group.The observation intervals were set at 2 h,6 h,24 h and 72 h after ACI (n =8,at each interval).ACI model was established in rats by free falling objects hitting skull.Plasma levels of cardiac troponin Ⅰ (cTnⅠ) and TNF-αwere determined by using enzyme -linked immunosorbent assay (ELISA).And the myocardial Na +-K+ -ATPase and Ca2+ -ATPase activity changes were determined by colorimetric method.Then the pathological change of myocardium with HE staining were observed under light - microscopy.Results Plasma cTnⅠ and TNF-α levels increased (P ＜ 0.05),while myocardial Na+-K+-ATPase and Ca2+- ATPase activity decreased in the rats with ACI (P ＜ 0.05 ); There was a negative correlation between myocardial Na + -K + -ATPase、Ca2 + -ATPase activity and plasma Ievels of cTnI ( r =0.357,r =0.557,P ＜ 0.05),and positive correlation between plasma levels of TNF-α and cTnⅠ ( r =0.920,P ＜ 0.05 ).Vacuolar degeneration and necrosis with inflammatory cell infiltrates of myocardium were observed under light -microscopy.Conclusions ACI can lead to myocardial injury,resulting in increase in plasma cTnⅠ and TNF-αand decrease in myocardial ATPase activity.Decrease in myocardial ATPase and increase in TNF-αmay involve in the pathogenesis of myocardial injury after ACI.
