Determination of benzylacetone in the rhododendron by HPLC
10.3760/cma.j.issn.1673-4246.2012.11.014
- VernacularTitle:HPLC法测定烈香杜鹃中苄基丙酮的含量
- Author:
Fuqin ZHANG
;
Mei JIN
;
Lanlan LI
- Publication Type:Journal Article
- Keywords:
Benzylacetone;
Rhododendron;
HPLC
- From:
International Journal of Traditional Chinese Medicine
2012;34(11):1004-1006
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC method for determination of benzylacetone in the rhododendron.Methods The HPLC analysis was carried out on Luna C18(250 mm×4.6 mm,5 μm) at 30℃temperature.The mobile phase was acetonitrile-water (64 ∶ 36),the flow rate was 1 ml/min,and the detection wavelength was 220 nm.Results The linear range of benzylacetone was 17.08～170.8 μg/ml (R=0.9998),the average recovery of this method was 99.81％,and the RSD was 1.46％.Conclusion The method is convenient,quickly and accurate,can be used for the quality control of rhododendron oil and their preparations.
