MicroRNA profiles in pancreatic ductal adenocarcinoma
10.3760/cma.j.issn.1674-1935.2012.05.007
- VernacularTitle:胰腺导管腺癌microRNA表达谱的研究
- Author:
Jing ZHANG
;
Chenyan ZHAO
;
Qinghua LIU
;
Danghui YU
;
Ying CHEN
;
Min SHI
;
Canrong NI
;
Minghua ZHU
- Publication Type:Journal Article
- Keywords:
Pancreatic neoplasms;
MicroRNA;
Microarray analysis;
Gene expression profiling
- From:
Chinese Journal of Pancreatology
2012;12(5):313-316
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the differentially expressed microRNA (miRNA) between pancreatic ductal adenocarcinoma (PDAC) and para-cancerous tissues,and determine related target genes.Methods Nine fresh PDAC tumor tissues and 3 adjacent normal pancreatic tissues were collected,then Agilent miRNAmicroarray with 713 miRNA loci was used to identify the differentially expressed miRNA.Real-time PCR method was applied to verify the up-regulated miRNA.TargetScan 5.1 and miRandaV5 software were used to analyze the related target genes.Results miRNA microarray identified 11 PDAC related miRNAs,among them,the expressions of miR-194*,miR-192*,miR-602,miR-194 were up-regulated,while the expressions of miR-139-3p,miR-513a-5p,miR-630,miR-30c-1 *,miR-887,miR-508-5p,miR-516a-5p were down-regulated.The expressions of miR-192,miR-194 and their homolog were verified in 31 PDAC tumor tissues.After software analysis,it was found that target genes of miR-192 were ZEB2,CXCL-2,EEF1A1,ERCC3,and target genes of miR-192 * were DCC,SMAD4,FAS,and target genes of miR-194 included DACHI,IGSF11,PTPN2,RBBP4,while target genes of miR-194 * included CD40LG,CIDEB,FHL1.Conclusions Eleven differentially expressed miRNAs are present in PDC,and they may be involved in the occurrence and development of PDC.
