Expression of DNMT3b and microRNA-29b in pancreatic cancer cells and their ralationship

VernacularTitle:DNA甲基转移酶3b和microRNA-29b在胰腺癌细胞株中的表达及其相关性
Author: Lihua WANG ; Jun GAO ; Yiqi DU ; Hongyu WU ; Jing JIN ; Shude LI ; Zhaoshen LI
Publication Type:Journal Article
Keywords: Pancreatic neoplasms; Cell line,tumor; DNA-methyltransferases3b; MicroRNAs
From: Chinese Journal of Pancreatology 2012;12(5):317-319
CountryChina
Language:Chinese
Abstract: Objective To investigate the expression of DNMT3b mRNA and microRNA-29b (miR-29b) in pancreatic cancer cells and analyze their relationship.Methods Real-time RT-PCR method was used to detect the expression of DNMT3b mRNA and miR-29b in five pancreatic cancer cell lines ( PANC1,BxPC3,CFPAC,AsPC 1,Capan 2 ) and the relationship between DNMT3b mRNA and miR-29b expression was analvzed by pearson linear correlation method.Results The expression of DNMT3b mRNA in PANC1,BxPC3,CFPAC,AsPC 1,Capan 2 were 0.497 ±0.184,0.420 ±0.168,0.439 ±0.217,0.122 ±0.111 and 0.731 ±0.387,while the expression of miR-29b were 0.745 ± 0.596,0.797 ± 1.000,0.464 ± 0.430,1.836 ± 1.623 and 0.216 ± 0.335.The expression of DNMT3b mRNA was negatively correlated with the expression of miR-29b (r =-0.922,P =0.026 ).Conclusions Both DNMT3b and miRNA29b are involved in the carcinogenesis of pancreatic cancer,and they are negatively correlated.