Reactive oxygen species(ROS) involving in inhibiting proliferation of human bone marrow neuroblastoma cells (SH-SY5Y) by giant typhonium rhizome water extract
10.3760/cma.j.issn.1673-4246.2012.10.012
- VernacularTitle:活性氧对白附子水提物抑制人骨髓神经母细胞瘤细胞增殖作用的影响
- Author:
Tongzhang YANG
;
Hongbo GAI
- Publication Type:Journal Article
- Keywords:
Giant typhonium rhizome;
Reactive oxygen species;
Human bone marrow neuroblastoma cells;
Flow cytometry
- From:
International Journal of Traditional Chinese Medicine
2012;34(10):898-900
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate whether the reactive oxygen species involved in inhibiting proliferation of human bone marrow cells of neuroblastoma cells (SH-SY5Y)process by giant typhonium rhizome water extract,and to investigate the initial mechanism of anti-tumor effect of giant typhonium rhizome in vitro.MethodsAfter giant typhonium rhizome water extract processing of human bone marrow neuroblastoma cells (SH-SY5Y)at 24 h and 48 h,the cells were collected,and western blotting reaction methods such as flow cytometry was used to study the levels of ROS and SH-SY5Y apoptosis.ResultsThe levels of ROS elevated from before dosing (4.9±1.6)％ to (39.7 ± 11.8)％ in the process of giant typhonium rhizome water extract induced apoptosis in SH-SY5Y; after ROS inhibition was added,SH-SY5Y apoptosis level induced by giant typhonium rhizome water extract had decreased.ConclusionROS involved in the process of giant typhonium rhizome water extract inhibiting cell proliferation in SH-SY5Y.
