Detection of IL-2 and IL-6 in human breast tumor Bcap-37 cells treated by hematoporphyrin nonomethyl ether mediated photodynamic therapy
10.3760/cma.j.issn.1006-9801.2012.08.009
- VernacularTitle:血卟啉单甲醚介导的光动力作用后人类乳腺癌Bcap-37细胞白细胞介素-2和白细胞介素-6的检测分析
- Author:
Lihua LIU
;
Minghui HUANG
;
Yanchun QIAN
;
Hongbo ZHANG
- Publication Type:Journal Article
- Keywords:
Breast neoplams;
Interleukin-2;
Interleukin-6;
Hematoporphyrin nonomethyl ether mediated photodynamic therapy
- From:
Cancer Research and Clinic
2012;24(8):530-532
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe IL-2 and IL-6 changes in the breast tumor Bcap-37 cells reated by hematoporphyrin nonomethyl ether mediated photodynamic therapy (HMME-PDT).Methods Cells in logarithmic growth phase were collected among breast cancer cells cultured in conventional methods.According to blank control group or the experimental group (laser irradiation group,photosensitive agent group and HMME-PDT group),PDT in addition to HMME and HMME-PDT were conducted.The changes of IL-2,IL-6 were detected by radioimmunoassay.Results After HMME-PDT,IL-2 was increased as time passed.After 12,24 and 48 h,compared with IL-2 level in the control group,in laser irradiation group or photosensitive agent group,the levels of IL-2 in HMME-PDT group was significantly differences (P ＜ 0.05).But IL-6 levels decreased.The most obvious changes of IL-6 levels happened at 12h and 24h.There was significant differences between IL-6 in HMME-PDT group with the control group,laser irradiation group or photosensitive agent group (P ＜ 0.05).Conclusion HMME-PDT maybe have destruction effect by altering IL-2,IL-6 activity on breast tumor cells,which provides objective indicators for clinical patients to regulate immune function and auxiliary diagnosis.
