Study on the resistant genes and virulence gene of vancomycin-resistant Enterococci
10.3760/cma.j.issn.0254-5101.2012.09.002
- VernacularTitle:耐万古霉素肠球菌耐药基因及毒力因子研究
- Author:
Yamin LU
;
Xiaosi LI
;
Qing WU
;
Anqing ZOU
;
Tieli ZHOU
;
Xiaolei ZHANG
;
Jianming CAO
- Publication Type:Journal Article
- Keywords:
Enterococci;
Vancomycin-resistant Enterococci;
Virulence gene;
Multilocus sequence typing
- From:
Chinese Journal of Microbiology and Immunology
2012;32(9):765-769
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the vancomycin-resistant genes and the virulence factors genes in vancomycin-resistant Enterococci (VRE),and to analyze the drug-resistance character and epidemic characteristics of VRE strains and provide the basis for clincal selection of drugs and infection control.Methods VRE were screened by agar dilution sieving plate (ADSP) containing 6 μg/ml of vancomycin,drug resistance of VRE to other common antibiotics were detected by VITEK-60 automatic microbial analyzer.The gene types and virulence factor genes of VRE were determined by PCR.And the genetic relationships among VRE were determined by multilocus sequence typing.Results Seven vancomycin-resistant Enterococcus faecium strains were found in 360 enterococcus strains.All the VRE strains exhibited high-level vancomycin resistance ; some of them were medium or senstive to teicoplanin.They all carried vanA gene and esp gene and one of them carried 4 kinds of virulence factor genes.The ST type of the 7 VRE strains were diffused distribution.Conclusion We found vanB phenotype vanA genotype vancomycin-resistant Enterococcus faecium isolates in Wenzhou; these VRE strains were multidrug resistance and carried various virulence factor genes.Linezolid could be used as a recommend drug for treatment of VRE infection.The protection of antibiotics sensitivity should be strengthened.
