Differentially expressed genes associated with apoptosis in human rhabdomyosarcoma cells infected by enterovirus 71
10.3760/cma.j.issn.0254-5101.2012.08.011
- VernacularTitle:肠道病毒71型感染人横纹肌肉瘤细胞不同时期凋亡相关基因差异表达研究
- Author:
Xiang LI
;
Xueling HOU
;
Hongjun PENG
;
Mei SHI
;
Qingbo JIANG
;
Xiping LIU
;
Yuhua YAO
;
Caizhen HE
;
Weifeng SHI
- Publication Type:Journal Article
- Keywords:
Enterovirus 71;
RT-PCR array;
FasL;
CD40L;
Apoptosis
- From:
Chinese Journal of Microbiology and Immunology
2012;32(8):718-724
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the different apoptotic gene expressions and apoptotic signaling transduction of human rhabdomyosarcoma (RD) cells infected by enterovirus 71 (EV71) in different stage.Methods The survival of EV71-infected RD cells was observed by trypan blue staining.The apoptotic morphology and rates of RD cells were surveyed and detected by Annexin V-FITC/PI staining and flow cytometry,respectively.PCR array was employed to analyze 88 apoptotic gene expressions from EV71-infected RD cells at 8 h and 20 h postinfection (p.i),respectively.Results After RD cells were infected with EV71 (MOI =5) at 8 h p.i,the viability was significantly decreased.Flow cytometry data demonstrated that the apoptotic rates of EV71-infected RD cells had increased to 18.0％ and 19.1％ at 20 h p.i in early and later stage respectively.RT-PCR array studies revealed significant variations in the expression of apoptotic genes.Among 88 genes,only the expression of IFN-β1 was upregulated 5.22 folds,whereas 47 genes including ACIN1,Akt,Apaf1,caspase and CIDEB were found to be downregulated that were lower than 2 folds at 8 h p.i.However,28 genes including FasL,CD40L,TNF,caspase-10 and caspase-3 were induced more than 2 folds after EV71 infection at 20 h.Conclusion The downregulation of apoptosis-related genes is associated with viral apoptosis-suppressing effect in RD cells in the early stage of EV71 infection.The death receptor signaling pathways including Fas/FasL and TNF/TNFR are activated to induce cell apoptosis in the late stage of EV71 infection.Moreover,host cell can also inhibit apoptosis by regulating signal pathway of CD40/CD40L,NF-κB/RelA and PI3K/Akt activation.
