Screening of HIV-1 neutralizing antibody mimotopes in slow progressor from phage display peptide library
10.3760/cma.j.issn.1009-9158.2012.09.015
- VernacularTitle:应用噬菌体随机肽库技术筛选缓慢进展者HIV-1中和抗体模拟表位
- Author:
Xiaoli ZHANG
;
Xiaoxu HAN
;
Di DAI
;
Mingjia BAO
;
Zining ZHANG
;
Min ZHAO
;
Hua NIAN
;
Hong SHANG
- Publication Type:Journal Article
- Keywords:
HIV-1;
Epitopes;
Peptide library;
HIV infections;
Antibodies,neutralizing
- From:
Chinese Journal of Laboratory Medicine
2012;35(9):838-842
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo screen mimetic HIV-1 neutralizing epitopes from plasma with high level neutralizing antibody,and to provide useful information for further study of the interaction between antigen and antibody.MethodsIn order to gain neutralizing antibody recognized mimotopes, we detected neutralizing antibodieslevelsof 11HIV-1infectedslowprogressorsbyPBMC-basedneutralization assays.High-titer HIV-neutralizing antibodies from plasma of SPs was used as the ligand for biopanning by phage-displayed random peptide library.Positive phage clones was evaluated by ELISA,sequenced,and analyzed for homology to HIV-1 env by local BLAST to deduce the neutralizing peptide.ResultsTwenty-two clones were obtained consistent with requirement through three rounds biopanning.After comparison analysis,twelve clones include C8 were obtained as mimotopes of neutralizing antibody,C40 located in gp41Ⅱ cluster with the highest titer by inhibition ratio may be as neutralizing epitope.Conclusion By the use of IgG antibodies from SPs to screen the phage random polypeptide library,one can acquire multiple phage mimetic peptides of HIV related antigen epitope.(Chin J Lab Med,2012,35:838-842 )
