Effect of high glucose on the expression of activating transcription factor 4 in cultured retinal Müller glia cells
10.3760/cma.j.1005-1015.2012.05.016
- VernacularTitle:高糖对体外培养的视网膜Müller细胞表达蛋白转录活化因子4的影响
- Author:
Jing GUO
;
Min KE
- Publication Type:Journal Article
- Keywords:
Diabetic retinopathy/physiopathology;
Endoplasmic reticulum/physiopathology;
Proteins;
Animal experimentation
- From:
Chinese Journal of Ocular Fundus Diseases
2012;28(5):498-500
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of high glucose on the expression of activating transcription factor 4 (ATF4) in cultured retinal Müller glia cells.Methods The retinal tissue of Sprague Dawley (SD) rats was collected,and Müller cells were isolated and cultured.The glial fibrillary acidic protein (GFAP) and glutamine synthetase (GS) of Müller cells were identified by streptavidin-biotinperoxidase complex.Cultured rat Müller cells were divided into control group (5.5 mmol/L glucose),group A (20 mmol/L glucose),group B (30 mmol/L glucose) and group C (40 mmol/L glucose).ATF4 protein expressions in Müller cells of four groups were measured by Western blot four days after cultured.Results GFAP and GS expressed in more than 95％ of Müller cells.Over 95％ of Müller cells of group A,B and C were positive for GFAP and GS.Western blots indicated that ATF4 protein in group A,B and C increased obviously compared with the control group (q=0.293,0.754,0.484; P＜0.05).Conclusion High glucose can increase the expression of ATF4 protein and cause endoplasmic reticulum stress in retinal Müller glia cells in vitro.
