Human carcinoembryonic antigen-related cellular adhesion molecules 1 mediated susceptibility of Neisseria gonorrhoeae to mouse

VernacularTitle:人癌胚抗原相关细胞黏附分子1介导的小鼠淋病奈瑟菌易感性
Author: Guocai LI ; Qinghui DU ; Xiaohong WANG ; Jinsong WANG ; Feng YU ; Shuangxi LIU ; Rushan XIE ; Hongmei JIAO ; Hua YAN ; Mingchun JI
Publication Type:Journal Article
Keywords: hCEACAM1; Transgenic mice; Neisseria gonorrhoeae; Susceptibility
From: Chinese Journal of Microbiology and Immunology 2011;31(10):907-911
CountryChina
Language:Chinese
Abstract: ObjectiveTo develop a transgenic mouse model for N.gonorrhoeae researches.Methods Human carcinoembryonic antigen-related cellular adhesion molecules 1 (hCEACAM1) eukaryotic expression vector,pCDPGICAM1,was used to generate transgenic mice by microinjection.The funder mice were screened by PCR,sequence analysis,Western blot and fluorescence-activated cell sorting analysis,respectively.The transgenic mice expressing hCEACAM1 were inoculated with N.gonorrhoeae intravaginally.Adhesion and infection of gonococci to mice were analyzed by bacteria culture and microscopy.Results Four (lines 50,53,54,and 59) of the 22 F0 generation transgenic mice were found to carry the transgene.The hCEACAM1 protein was expressed on the cell membrane of various tissues in the line 53 transgenic mouse.Compared with normal mice,N.gonorrhoeae can successfully infect and cause inflammation in the transgenic mice.Conclusion The hCEACAM1 transgenic mouse can be used as an animal model for gonococcal infections.