Effect of EDTA combined with ciprofloxacin on Pseudomonas aeruginose biofilm infection in guineapig

VernacularTitle:依地酸与环丙沙星联合应用对豚鼠铜绿假单胞菌生物被膜感染模型的作用
Author: Yayin LIN ; Jialin YU ; Qi LU ; Lihua LIN
Publication Type:Journal Article
Keywords: Pseudomonas aeruginosa; Biofilm; Ethylenediamine tetraacetic acid
From: Chinese Journal of Microbiology and Immunology 2011;31(10):912-915
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect of EDTA combined with ciprofloxacin on Pseudomonas aeruginose biofilm in vivo.Methods Pseudomonas aeruginosa was inhaled into the lung of guinea pigs and colonized,formed biofilm.After 7 days,the model was treated with ciprofloxacin,EDTA alone,or a combination of both for 7 days.The number of colony in the lungs is measured by agar plate.The pathological change of the lung is observed by hematoxylin and eosin (HE) staining and scanning electron microscope.Results EDTA combined with ciprofloxacin make the number of bacteria in the lungs reduced from l05 CFU/g to 10 CFU/g(t =24.67,P＜0.05),the lung lesion was less-sever histophathologically.Conclusion The combination of EDTA with ciprofloxacin has significant activity to remove mucoid PA biofilm in vivo.