Effect of TLR4/PI3K-related signaling molecules on the migration of asthmatic airway smooth musde cells induced by airway epithelial cells
10.3760/cma.i.issn.0254-5101.2011.11.010
- VernacularTitle:TLR4/PI3K信号相关分子在气道上皮细胞诱导的哮喘气道平滑肌细胞迁移功能中的作用
- Author:
Biwen MO
;
Haiying SU
;
Jianhong WEI
;
Jianwei HUANG
;
Changming WANG
;
Jinrong ZENG
;
Oing XU
;
Yun LIN
- Publication Type:Journal Article
- Keywords:
TLR4;
PI3K;
Airway smooth muscle cell;
Epithelial cells;
Cell migration;
Airway remodeling;
Bronchial asthma
- From:
Chinese Journal of Microbiology and Immunology
2011;31(11):994-999
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the effect of TLR4,PI3K and NF-κB on the migration of asthmatic airway smooth muscle cell(ASMCs) induced by airway epithelial cells.MethodsPrimary ASMCs were cultured by the method of cell digestion.Cell culture supernatant of RTE cells were collected by TNF-α stimulation of epithelial cells.Detected the IL-8 and RANTES levels in the supernatant.The effect of TLR4/PI3K-related signaling molecules on the migration of asthmatic ASMCs induced by epithelial cells were detected by Modified Boyden chemotaxis chamber with anti-TLR4 antibody,Wortmannin and PDTC drugs as a tool.ResultsThe levels of IL-8 and RANTES in the supernatant of TNF-αgroups were significantly increased,and that in the 20 ng/ml group was significantly higher than other groups ( P＜0.01 ).Compared with control group,the transmembrane migration of asthmatic ASMCs from other groups was significantly increased (P＜0.01 ).The transmembrane migration of asthmatic ASMCs from treated groups was significantly increased than asthma group (P＜0.01).The migration of asthmatic ASMCs from TNF-α+anti-TLR4 antibody group,TNF-α+Wortmannin group and TNF-α+Wortmannin+PDTC were significantly decreased than that of TNF-αgroup( P＜0.01 ).The migration of asthmatic ASMCs from TNF-α+Wortmannin+PDTC were significantly decreased than that of TNF-α+Wortmannin group (P＜0.05).ConclusionTLR4/PI3K-related signaling molecules involved in the transmembrane migration of asthmatic ASMCs induced by airway epithelial cells and may be one of the mechanisms of airway remodeling of asthma.
