Amelioration of experimental autoimmune myocarditis by HVEM-overexpressing dendritic cells through induction of IL-10-producing cells
10.3760/cma.j.issn.0254-5101.2011.11.014
- VernacularTitle:HVEM过表达树突状细胞对自身免疫性心肌炎的作用
- Author:
Gang CAI
;
Huaizhou WANG
;
Beiying WU
;
Jiafei LIN
;
Qian SHEN
- Publication Type:Journal Article
- Keywords:
Experimental autoimmune myocarditis;
Hepers virus entry mediator;
Immune regulation;
Dendritic cells
- From:
Chinese Journal of Microbiology and Immunology
2011;31(11):1017-1022
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo assess the efficacy of herpes virus entry mediator (HVEM) gene modifled dendritic cells (DCs) in protecting against myosin induced myocarditis,and to investigate the involving mechanism.MethodsWe treated experimental autoimmune myocarditis (EAM) mice with myosin-pulsed DCs which were transfected with HVEM-expressing adenovirus (Ad-HVEM) or control vectors,then evaluated myocarditis,plasm cTn [ and autoantibody by histopathology,fluoroimmunoassay,and ELISA,respectively.ResultsWe found that DCs transfected with Ad-HVEM (DC-Ad-HVEM) could protect against EAM.Further study showed DC-Ad-HVEM could produce regulatory cytokine IL-10,and IL-10 promoted the production of a key regulatory T cell subset which is important in peripheral tolerance.The T cells mediated protection against EAM.ConclusionThis study suggest that myosin-DC-Ad-HVEM cell gene therapy is a safe and effective way for inhibiting the development of EAM,and the signal net mediated by HVEM plays different roles in different cells.
