Study on HBV inhibits the expression of complement 3 and complement 4 both in vivo and in vitro

VernacularTitle:乙型肝炎病毒在体内外抑制补体C3和C4表达的研究
Author: Xinghui LIU ; Xing ZHOU ; Hui SONG ; Chengliang ZHU ; Fang LIU
Publication Type:Journal Article
Keywords: Hepatitis B virus; Gene chip; Complement component 3; Complement component 4
From: Chinese Journal of Microbiology and Immunology 2011;31(10):927-930
CountryChina
Language:Chinese
Abstract: Objective To explore the effect of hepatitis B virus(HBV) on the expression of complement 3 (C3) and complement 4 (C4) and its regulatory mechanism.Methods Differentially expressed genes between HepG2 and HepG2.2.15 cells was screened by gene chip,serum complement component 3 (C3) and 4 (C4) levels in patients with HBV infection and in healthy individuals were measured by Immunoturbidimetry,HBV infectious clone pHBV1.3 was transfected into HepG2 cells,and expression of C3 and C4 was measured by RT-PCR and Western blot.Results Expression of C3 and C4 mRNA was lower in HepG2.2.15 cells than in HepG2 cells,serum C3 and C4 levels was much lower in patients with chronic hepatitis B and hepatic carcinoma as compared to healthy individuals (P＜0.05 ).HBV could downregulate the expression of C3 and C4 at mRNA and protein levels.Conclusion HBV may inhibit the expression both in vivo and in vitro.