The effect of adenovirus mediated LIF and OSM co-expressing on proliferation and differentiation of umbilical cord blood CD34+ hematopoietic stem/progenitor cell
10.3760/cma.j.issn.0254-5101.2011.10.001
- VernacularTitle:Ad-LIF-OSM双基因转染饲养细胞对脐血造血细胞体外增殖和分化的影响
- Author:
Wanrong BAO
;
Xin YU
;
Weihua SHENG
;
Fengjuan ZHANG
;
Jiarong WANG
;
Jicheng YANG
;
Jingcheng MIAO
- Publication Type:Journal Article
- Keywords:
Adenovirus;
Leukaemia inhibitory factor (LIF);
Oncostain M ( OSM );
Co-expressing vector;
Hematopoietic stem/progenitor cell(HSPC)
- From:
Chinese Journal of Microbiology and Immunology
2011;31(10):865-869
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish Ad-LIF-OSM transgenic feeder cells for the expansion of CD34+ hematopoietic stem/progenitor cell and tentatively study its effect in expansion and differentiation of cord blood hematopoietic stem cell(HSC) in vitro.Methods In the foundation of pAdTrack-CMV-LIF-polyA+promoterΔ,the OSM gene was inserted to the vector plasmid.Then we structure the transfer plasmid pAdTrack-CMV-LIF-polyA+promoterΔ-OSM.The transfer vector and backbone vector were further cotransfected for homologous recombination. The result pAdEasy-1-pAdTrack-CMV-LIF-polyA + promoterΔ-OSM homologous recombination plasmid were transfected into the human embryonic kidney 293 (QBI-293A) cells,leading to formation of the recombinant adenviruses Ad-LIF-OSM which co-expressing LIF and OSM.Infect the feeder layer cells with groups of Adenovirus,detection the expressing of LIF and OSM in WI-38 cells by RT-PCR and ELISA.Compares the stem cells differentiation and proliferation of the different experimental groups in vitro by transwell and cell counting.Results The sequencing results show that the OSM genes were anastomotic in Ad-LIF-OSM.LIF and OSM gene could be detected in feeder layer cells which infected by Ad-LIF-OSM.Exogenetic LIF and OSM have special effect in culturing HSC in vitro.Conclusion The adenoviral vector co-expressing LIF and OSM (Ad-LIF-OSM) were successfully constructed.Ad-LIF-OSM transgenic feeder cells can effectively proliferate umbilical cord blood CD34+ HSPC in vitro and delay it differentiate.
