Diagnostic value of detection of IgM antibodies to EV71-infection patients
10.3760/cma.j.issn.0254-5101.2011.10.020
- VernacularTitle:IgM抗体检测对肠道病毒71型感染者诊断价值的探讨
- Author:
Bin WU
;
Liang LI
;
Fenyang TANG
;
Xian QI
;
Rongqiang ZU
;
Lunbiao CUI
;
Fengcai ZHU
;
Minghao ZHOU
;
Hua WANG
- Publication Type:Journal Article
- Keywords:
Enterovirus 71;
IgM antibody;
Neutralization test;
Real-time PCR
- From:
Chinese Journal of Microbiology and Immunology
2011;31(10):934-937
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the diagnostic value of detection of IgM antibodies to EV71-infection patients,and compared characterisation of RT-PCR,IgM capture ELISA and neutralization test.Methods Virus RNA,neutralization titer and IgM antibody in 115 EV71-infection patients were detected by EV71 real-time RT-PCR kit( EV71-PCR kit),neutralization test,and EV71 IgM-capture ELISA kit (EV71-IgM kit),respectively.Results Using EV71-IgM kit,the detection rate was 80.9％ (93/115,95％ CI:72.5-87.6) among the 115 EV71-infection patients,and was 2.6％ among the 228 healthy children.Simultaneously,sera collected after 1-2 day of disease onset showed an IgM positivity of 70.4％ (38/ 54).The positive rate of EV71-PCR among these patients was 82.6％ (95/115,95％ CI:74.4-89.0),so there was no statistically significant differences between it and EV71-IgM kit.In addition,the detection rate in EV71-infection patients could increase to 92.2％ by combined detection of EV71-PCR and EV71-IgM kit.Conclusion EV71-IgM kit was a rapid and valuable way for the early diagnosis of EV71 infection,and could significantly improve detection rate for EV71 infection by combining with EV71-PCR kit.
