Effects of four extracts of Chinese herbal medicines on cellular immunity in rats induced by multiple drugs resistant bacillus tuberculosis among pneumoconiosis patients complicated with tuberculosis
10.3760/cma.j.issn.0254-5101.2011.10.008
- VernacularTitle:4种中药提取物对尘肺结核患者感染的耐多药结核分枝杆菌感染小鼠细胞免疫的影响
- Author:
Jun LU
;
Song YE
;
Yun DENG
;
Zhongchang HU
;
Rui QIN
- Publication Type:Journal Article
- Keywords:
Radix Ranunculi Ternati;
Radix Sophorae Flavescentis;
Prunella vulgaris L.;
Stellera chamaejasme L.;
Cytokine
- From:
Chinese Journal of Microbiology and Immunology
2011;31(10):893-897
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the regulative effect of the extract of Radix Ranunculi Ternati,Radix Sophorae Flavescenti,Prunella vulgaris L.and Stellera chamaejasme L.on cellular immunity induced by multiple drugs resistant bacillus tuberculosis(MDR-TB) from pneumoconiosis patients complicated with tuberculosis in rats.Methods MDR-TB model in rats was induced by MDR-TB.Normal control group were feed by standard feed,model group were irrigated by normal saline,and the other groups were respectively feed by the extract of Chinese herbal medicines.The content of IFN-γ,IL-4,IL-10 and IL-12 were examined by ELISA.RT-PCR was used to detect the mRNA levels of them.Results The content of IFN-γof the four extracts of Chinese herbal medicines were (2.01 ±0.73 ),( 1.92±0.56),( 1.98 ±0.67 ) and (1.94±0.59) pg/ml,IL-4 were (6.01±1.46),(6.12±1.35),(6.47±1.46) and (6.15±1.44) pg/ml,IL-10 were (12.09±3.07),( 12.45±4.01 ),( 12.13±3.43) and (12.54±3.78) pg/ml,IL-12 were (2.99±0.89),(2.75±0.84),(3.02±0.86) and (2.89±0.75) pg/ml.Compared to the model group,they resulted in significant in serum IFN-γ,IL-12,IL-4 and IL-10 (P＜0.05 or P＜0.01 ),the mRNA levels of them were significantly (P＜0.05 or P＜0.01 ).Conclusion The four extracts of Chinese herbal medicines can enhance the cellar immunological function in rats from up-regulation of the level of genetic transcription,accordingly provide the theory base of healing of pneumoconiosis patients complicated with tuberculosis with them.
