Identification of bacteria and fungi by matrix-assisted laser desorption ionization-time of flight mass spectrometry
10.3760/ama.j.issn.1009-9158.2011.11.007
- VernacularTitle:应用基质辅助激光解析电离飞行时间质谱鉴定常见细菌和酵母菌
- Author:
Mingxin ZHANG
;
Min ZHU
;
Mei WANG
;
Yinguang CAO
;
Xinxin LU
- Publication Type:Journal Article
- Keywords:
Bacteria;
Yeasts;
Spectrometry,mass,matrix-assisted laser desorption-ionization
- From:
Chinese Journal of Laboratory Medicine
2011;34(11):988-992
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify the pathogenic microorganism by MALDI-TOF MS.Methods A total of 560 strains were resuscitated,which included 260 gram-positive bacteria strains,180 gram-negative bacteria strains,60 yeast-like-fungi strains and 60 enteropathogenic bacteria strains.Comparing MALDI-TOF MS with Vitek2 Compact,the discordant results were validated by 16S rDNA sequencing.Results Comparing MALDI-TOF MS with Vitek2 Compact,the coincidence rate was 94.6％ (246/260) for gram-positive bacteria,96.7％ (174/180) for gram-negative bacteria,95％ (57/60) for yeast-like-fungi,and 93.3％(56/60) for enteropathogenic bacteria Fifteen strains were validated by 16S rDNA gene sequencing.Comparing with sequencing,the coincidence rate of two methods was 66.7％ ( 10/15 ) for MALDI-TOF MS and 26.7％(4/11 ) for Vitek2 Compact,respectively.Conclusion MALDI-TOF MS shows rapid turnaround time and modest reagent costs,and it will be another effective tool for microorganism diagnosis.
