Screening and verification of the siRNA targeted for the HOXA10 gene

VernacularTitle:靶向同源盒A10特异性干扰性小RNA序列的筛选及功能鉴定
Author: Xiuhong JIA ; Wenwen FAN ; Jianchang LI ; Shuyang XIE
Publication Type:Journal Article
Keywords: Neoplasms; Genes,homeobox; RNA interference; A549 cell
From: Cancer Research and Clinic 2011;23(11):743-746
CountryChina
Language:Chinese
Abstract: Objective To obtain effective siRNA fragment of HOXA10 gene and verify its function,to supply experimental evidence for tumor prevention and curation by RNAi targeting to HOXA10 gene.Methods Three pairs of small interfering RNA targeting to the different sites of HOXA10 were designed and introduced into A549.The mRNA expression of HOXA10 of A549 was detected by semi-quantitative RT-PCR,the cell proliferation was assayed by MTT,the apoptosis was measured by flow cytometry.The most effective siRNA assay was screened and was tested the relationship between it and proliferation and apoptosis.Results The mRNA of HOXA10 was inhibited by three siRNAs in A549 cells,among which siRNA1 gave the strongest inhibiting of HOXA10 by ODR was (20.190±1.698) ％.The inhibitory rate of cell proliferation was (69.793±2.092) ％ and the apoptosis rate was (29.593±2.670) ％.Conclusion siRNA1 can specifically degrade HOXA10 mRNA and inhibit the proliferation of A549 cell and promote its apoptosis.