Clinical application of circulating microRNA in laboratory medicine
10.3760/cma.j.issn.1009-9158.2011.10.001
- VernacularTitle:临床实验室检测循环微小RNA中应关注的问题
- Author:
Gusheng TANG
;
Qian SHEN
- Publication Type:Journal Article
- Keywords:
microRNAs;
Biological markers;
Quality control
- From:
Chinese Journal of Laboratory Medicine
2011;34(10):865-870
- CountryChina
- Language:Chinese
-
Abstract:
microRNA(miR) in body fluids shows good stability and the change in its expression profile and level is associated with cancer and other diseases.Thus,circulating miR has been proposed to be an useful biomarker in diagnosis of the onset,prognosis and risk of diseases.Although great discoveries have been obtained on all aspects of miR,there are some shortcomings existing in the present studies,such as inconsistent specimens,diverse detection methods,lack of standardization on data analysis and other defects,resulting in poor reproducibility of data and inconsistent clinical conclusion,that make circulating miR difficult to be used in clinical laboratory testing.So lots of work is needed for us to implement,such as standardizing sample selection,improving detection reagents and methods,and normalizing test results before we can ultimately establish a fast,easy,low-cost detection of circulating miR in the clinical laboratory.
