Genetic analysis of a partial VP1 region and molecular identification of non-EV71, non-CAl6 virus strains of hand, foot and mouth disease(HFMD) in Guangdong province between 2008 and 2009
10.3760/cma.j.issn.0254-5101.2011.09.013
- VernacularTitle:2008-2009年广东手足口病非CA16非EV71肠道病毒株的鉴定及其VP1基因分型研究
- Author:
Hong XIAO
;
Dawei GUAN
;
Hanri ZENG
;
Wei LI
;
Juan SU
;
Huanying ZHENG
;
Xue GUO
;
Leng LIU
- Publication Type:Journal Article
- Keywords:
Hand,foot and mouth disease;
Enterovirus;
VP1 gene;
Type
- From:
Chinese Journal of Microbiology and Immunology
2011;31(9):808-812
- CountryChina
- Language:Chinese
-
Abstract:
Objective To discuss the prevalence of non-EV71,non-CA16 virus strains of hand,foot and mouth disease(HFMD) in Guangdong province between 2008 and 2009,and analyze the genetic evolution of these non-EV71,non-CA16 virus strains.Methods Isolated viruses from stool samples collected from outpatient and in-patient cases of HFMD between 2008 and 2009 by human rhabdomyosarcoma(RD) cell and HEp-2 cell,cultures that exhibited a characteristic enterovirus cytopathic effect were evaluated by RT-PCR.Those strains which identified non-EV71,non-CA16 were analyzed by VP1 sequencing and then were identified by BLAST program.A phylogenetic tree was constructed using the Neighor-Joinning method in the MEGA 4.0 software.Results Twenty-two virus strains of non-EV71,non-CA16 were obtained,and nine of the twenty-two virus strains in 2008 were classified into CA2,CA4,and CB3 by BLAST; thirteen of the twenty-two virus strains in 2009 were classified into EV80,Echo13,Echo30,CBS,Echo24,CA10,CA6,and poliovirus 1 by BLAST.The honology of all strains was low,and all the strains belonged to CA,CB,Echoviruses,Enterovirus and poliovirus subgroup.Conclusion Except for EV71 and CA16 was a major causative agent in prevail of HFMD in Guangdong province between 2008 and 2009,there also existed other subgroup Enterovirus.The other twenty-two strains respectively belonged to CA,CB,Echoviruses,Enterovirus and poliovirus subgroup,and none of those strains was predominant.Muti-species Enterovirus occurred concomitantly.
