Preliminary study of diagnosis ofhepatocellular carcinoma by protein chip technique

VernacularTitle:蛋白质芯片技术应用于肝癌诊断的初步研究
Author: Yunpeng ZHANG ; Sutang GUO ; Fuhua WANG ; Yumei WU ; Ruihong YANG ; Chunyan WANG
Publication Type:Journal Article
Keywords: Liver neoplasms; Diagnosis; SELDI-TOF-MS
From: Cancer Research and Clinic 2011;23(10):667-670
CountryChina
Language:Chinese
Abstract: Obiective To screen serum biomarkers in patients with hepatocellular carcinoma (HCC)by SELDI-TOF-MS technique.Methods SELDI-TOF-MS technique and CM10 Protein Chip were used to detect serum protein patterns of 46 patients with primary hepatic carcinoma and 64 healthy persons.The different proteins were obtained by the Biomarker Wizard software between the patients and healthy persons.The best biomarker of primary hepatic carcinoma was selected by evaluating the sensitivity and specificity of the protein.Results 16 protein peaks were obviously different between the patients and the healthy persons (P ＜0.05).The protein peaks of m/z 6845.70 had the highest diagnosis value with a sensitivity of 89.1％ (41/46)and specificity of 87.5 ％ (56/64).This protein was likely to be a part of the immunoglobulin heavy chain variable region.Conclusion The protein of m/z 6845.70 is potential biomarkers for the diagnosis of HCC.SELDI-TOF-MS technique is a quick,simple,convenient and high through-put technology for diagnosis of hepatocellular carcinoma.