Effects of ischemic postconditioning and preconditioning against ischemic reperfusion injury of skeletal muscle
10.3760/cma.j.issn.1001-2036.2011.05.010
- VernacularTitle:缺血后处理和预处理对大鼠骨骼肌缺血-再灌注损伤的作用
- Author:
Wei QI
;
Kejun CHEN
;
Shilian KAN
;
Xin ZHAO
- Publication Type:Journal Article
- Keywords:
Ischemic reperfusion;
Skeletal muscle;
Ischemic postconditioning;
Ischemic preconditioning
- From:
Chinese Journal of Microsurgery
2011;34(5):382-385
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the protective effects of ischemic postconditioning and preconditioning against ischemic reperfusion injury of skeletal muscle.Methods According to different treatment methods on ischemia-reperfusion injury,forty Wistar rats were divided into I/R group,IPost group,IPC group,IPC + IPost group,control group.Using a rat amputation-like model,Wistar rats underwent temporary amputation at the level of the femur,excluding the femoral vessels.By measuring MDA,MPO,the extent of skeletal muscle infarction,protective effects of postconditioning and preconditioning,postconditioning combined with preconditioning were observed.Results In the Ipost group,IPC group,IPC + Ipost group,MDA and MPO at one hour of reperfusion and extent of muscle infarction at 6 hour of reperfusion was lower than group IR (P ＜ 0.05).In the Ipost group,MDA,MPO and extent of muscle infarction was similar to group IPC + Ipost; In the Ipost group,MDA and MPO was lower than group IPC,extent of muscle infarction was similar to group IPC.Conclusion Ischemic postconditioning at the beginning of reperfusion can protect skeletal muscle against ischemic reperfusion injury.Preconditioning also protect skeletal muscle against ischemic reperfusion injury,but preconditioning combined with postconditioning don't offer additional benefit over preconditioning or postconditioning alone.
