Effects of SPARC on expression of TGF-βl and collagen type Ⅰ in keloid fibroblasts
10.3760/cma.j.issn.1671-0290.2011.05.016
- VernacularTitle:富含半胱氨酸的酸性分泌糖蛋白对瘢痕疙瘩成纤维细胞β1转化生长因子和Ⅰ型胶原蛋白基因表达的影响
- Author:
Chun WANG
;
Xiaojing LI
;
Chaohua LIU
;
Xiaojuan WENG
;
Dongyue HAO
- Publication Type:Journal Article
- Keywords:
Keloid;
Secreted protein,acidi and rich in cysteine;
Real-time fluorescence quantitative reverse transcription-PCR;
Transforming growth factor-β1;
Collagen type Ⅰ
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2011;17(5):377-380
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of secreted protein,acidic and rich in cysteine (SPARC) on the expression of TGF-β1 and collagen type Ⅰ in cultured human keloid fibroblasts by real-time fluorescence quantitative RT-PCR.Methods In vitro keloid fibroblasts were stimulated by different concentrations of recombinant human SPARC,and with the control group for comparison,real-time fluorescence quantitative RT-PCR to detect expression of TGFβ1 and collagen type Ⅰ.Results Compared with the control group,the expression of TGF-β1 and collagen type Ⅰ was significantly increased in the experimental group.Conclusions SPARC could enhance the expression of TGF-β1 and collagen type Ⅰ in keloid fibroblasts significantly.
