The expression of microRNA-202 in multiple myeloma and its clinical significance
10.3760/cma.j.issn.1009-9158.2011.10.014
- VernacularTitle:多发性骨髓瘤患者外周血单个核细胞微小RNA-202的表达及意义
- Author:
Xia ZHANG
;
Xudong WANG
;
Xianjuan SHEN
;
Xinhua WU
;
Wei SHI
;
Wencai ZHU
;
Zhenzong LIU
;
Shaoqing JU
- Publication Type:Journal Article
- Keywords:
Polymerase chain reaction;
microRNAs;
Multiple myeloma
- From:
Chinese Journal of Laboratory Medicine
2011;34(10):931-934
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method of SYBR Green Ⅰ FQ-PCR for detecting the expression of miR-202 in peripheral blood mononuclear cells ( PBMC ) and analyze the expression of miR-202 and its clinical significance in MM.Methods Reverse transcription was performed with specific stemloop primer for miR-202,and then FQ-PCR was used to detected the expression of miR-202 in 21 MM patients and 20 healthy people.Data was presented as mean ± standard deviation ( (x) ± s ).Non-parametric Mann-Whitney test was used to analyze the difference between MM group and control group.In addition,1∶ 125 dilution of one test sample was detected by repeated 5 times,and the same sample was tested one time a day for 3 days,repeated 5 each time.The assessment of repeatability of measurements was done by calculating standard deviation and variation coefficient from threshold cycle (Ct).Results FQ-PCR detection of miR-202 in PBMC was amplified by the standard S-curve,with a single melting curve peak and no complex peak,which showed good specificity.The assay showed good reproducibility (intra-assay coefficient 1.2％ and inter-assay coefficient 3.2％ ) and high sensitivity ( 12.8 pmol/μ1).The expression of miR-202 in MM was 0.014 ( 0.007 - 0.221 ) and 1.844 ( 0.162 - 3.966 ) in normal controls.The expression level of miR-202 was significantly higher in MM than in normal controls (U =48.000,P ＜0.01 ).Conclusions FQ-PCR provide us a rapid,sensitive and specific method for detection of miR-202.The expression level of miR-202 is higher in MM than in normal controls.It is possible to play a role in MM progress and may be a useful marker to evaluate the development and treatment of MM.
