Regulation on function and maturation of dendritic cells from systemic lupus erythematosus patients by triptolide
10.3760/cma.j.issn.0254-5101.2011.09.018
- VernacularTitle:雷公藤内酯醇对系统性红斑狼疮患者DC细胞功能及成熟的影响
- Author:
Limin LIU
;
Wenjing JIAO
;
Xingxia ZHANG
;
Yanming ZHANG
;
Guangsheng ZHAO
;
Yumei SUN
;
Shuhua LU
- Publication Type:Journal Article
- Keywords:
Triptolide;
Systemic lupus erythematosus;
Dendritic cells;
Maturation
- From:
Chinese Journal of Microbiology and Immunology
2011;31(9):824-829
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of immunomodulatory activity of triptolide on systemic lupus erythematosus(SLE) patients peripheral blood mononuclear cells( PBMC)-derived dendritic cells (DCs).Methods SLE -derived DCs were sorted by flow cytometry from peripheral blood mononuclear cells,then incubated with triptolide (0,5,10,30 μg/L ).After 24 h,we collected the supernatants and then detected the concentration of IFN-α,IL-6,TNF-α using ELISA.After 5 d,the cultrural cells were collected and CD11c,CD80,CD86 expression of DCs were analyzed by flow cytometry,we observed the morphology of DC by light microscope and electron microscope scanning.Results Triptolide -treated DCs from SLE patients with active and stable disease secreted lower level of IFN-α,IL-6,TNF-α,triptolide could inhibit DCs differentiation,which displayed more immature morphology and immunophenotypes than untreatedDCs.Conclusion Triptolide could decrease the immune function of DCs from SLE,inhibit differentiation and maturation of DCs.
