Apoptosis of hypertrophic scar fibroblasts induced by HMME-PDT

VernacularTitle:血卟啉单甲醚-光动力疗法诱导瘢痕成纤维细胞凋亡
Author: Cai HONG ; Gu YING ; Zeng JING ; Zhao GUANG
Publication Type:Journal Article
Keywords: Hematoporphyrin monomethyl ether; Hypertrophic scar fibroblast; Photodynamic therapy; Apoptosis
From: Chinese Journal of Medical Aesthetics and Cosmetology 2011;17(5):381-384
CountryChina
Language:Chinese
Abstract: Objective To investigate the apoptotic effects of hypertrophic scar fibroblast (HSF) induced by HMME-PDT.Methods Fibroblasts were cultured from nontreated hypertrophic scars,and cells at passages 4-6 were used for the experiments (photosensitizer dose 4 μg/ml,λ630 nm,pow er density 10 mw/cm2,energy fluence 2.5 J/cm2).Morphological and biochemical changes in fibroblasts were assessed by Hoechst 33258 staining and fluorescence microscopy.The rate of apoptotic or necrotic cells was detected by flow cytometry (FCM) through double staining of Annexin V -FITC and popodium iodide (PI),respectively.Results Marked morphological features of cell apoptosis were viewed under the fluorescent microscope through Hoechst 33258 staining.The analysis of FCM indica ted that the apoptotic rate was significantly increased after HMME PDT [(34.82 ± I.42) ％ vs (3.12±0.28) ％,P＜0.05],and apoptotic rate was higher than necrosis rate [(14.65±1.02) ％ vs (34.82±1.42) ％,P＜0.05].Conclusions Low level exposure to 630 nm PDT mediated by HMME appears to induce fibroblast apoptosis.