Research of mechanism about enhancing the activity of γδδT cells treated by resveratrol on colonic cancer cell lines SW-1116
10.3760/cma.j.issn.0254-5101.2011.08.008
- VernacularTitle:白藜芦醇对γδT细胞杀伤结肠癌SW-1116细胞的影响及机制研究
- Author:
Yuting JIANG
;
Sujuan FEI
;
Fuxing CHEN
;
Junquan LIU
;
Ling CHEN
;
Yi LI
;
Zhengzhong TAO
- Publication Type:Journal Article
- Keywords:
Resveratrol;
Colon cancer cells;
γδT cells
- From:
Chinese Journal of Microbiology and Immunology
2011;31(8):697-701
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of γδT cells on colonic cancer cell lines SW-1116 treated by resveratrol. MethodsAmplification γδT cells of human peripheral blood in vitro by using isopentenylpyrophosphate. Different density resveratrol induced γδT cells and colonic cancer cell SW-1 116, methyl thiazolyl tetrazolium(MTT) detectde the effect of growth and proliferation when expression veratrol action to γδT cells and colonic cancer cell lines SW-1116. Flow cytometer( FCM )detectde the expression of porforin, granzymeB and CD107a on γδT cells before it treated by resveratrol and after that. γδT cells against colonic cancer cell lines SW-1116 were detectde by lactate dehydrogenase(LDH) releasing assay before and after treated by resveratrol.Western blot analyzed the liveness of extracellular signal-regulated kinase1/2 of γδT cells before it treated by resveratrol and after that. ResultsγδTcells would be proliferation when the density of resveratrol in 0.39-3.125 μmol/L, while the effect on colonic cancer cell lines SW-1116 was not significance. There was significant difference of the expression of porforin, ranzymeB and CD107a on γδT cells before it treated by resveratrol and after that ( P＜0.05 ). γδT cells against colonic cancer cell lines SW-1116 treated by resveratrol was enhanced compared with control group( P＜0.05 ) . The p-ERK 1/2expression of γδT cells enhanced when the density of resveratrol treated to γδT cells in 0.1-10 μmol/L( P＜0.05 ). Conclusion Resveratrol could promote γδT cells, proliferation and strengthen the cytotoxicity of γδT cells against colonic cancer cell SW-1116, the mechanism might concerned with activating p-ERK1/2 and enhancing the expression of porforin, granzymeB and CD107a on γδT cells.
